Effect of mevalonate, zoledronate and BCG on monocyte/macrophage phenotype

Cells of innate immunity, mainly monocytes/macrophages, form a long-term nonspecific immunological memory during the initial encounter with pathogen, so-called trained immunity. Mevalonate pathway metabolites play an important role in formation The aim this investigation was to study effect modulators mevalonate pathway, and zoledronate, on immunity human animal monocytes/ macrophages. Material methods . Human monocyte-like cell lines THP-1 U-937, peritoneal macrophages BALB/c mice were used. Trained induced vitro by incubation U-937 for 24 72 hours inactivated Mycobacteria BCG vaccine strain, vivo intraperitoneal administration isolation day 7 after infection (lag phase). Cell hyperreactivity assessed response second stimulus bacterial lipopolysaccharide (LPS) mevalonate, zoledranate presence or absence LPS. Lactate, cytokine (IL-1β, TNF-α, IL-10), nitric oxide glucose level measured conditioned media from cells. Results discussion showed that responded differently production, lactate, consumption lag phase. zoledronate alone combination LPS also stimulated production different ways. phase cells is essential consumption. Peritoneal have been shown enhance pro-inflammatory LPS, zoledronate. Conclusions induce monocytes/macrophages.